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*Agricultural
Research, Extension, and Education Reform Act of 1998
Table of Contents
Cooperative Extension Programs
Cooperative Research Programs
Cooperative Extension Programs
GOAL 1: An Agricultural System that is Highly
Competitive in a Global Economy
Programs conducted under this goal addressed the following key
themes: Agricultural Profitability, Small Farm Viability, and Agricultural
Competitiveness.
Small Farm Family Program
The Program continued to meet the informational and educational
needs of the small family farmers in 22 southern counties of the state. To
accomplish the performance goal, assistance was given by providing written and
oral information in the following program areas:
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Insect-pests on fruit trees and control measures
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Living and working with physical disability
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Preparing "show" animals for competition
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Feeding and marketing of goats
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Breeding and raising dogs for supplemental income
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Community gardening for the low income families
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Insect control for vegetable gardens
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Weatherization of homes
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Health care information
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Canning and freezing of garden produce
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Pasture improvement
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Animal health
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Selling garden-fresh fruits and vegetables through farmers'
markets
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Advised on adding values to farm products before selling
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Forming a Beekeepers' Association
The above list is not meant to be exhaustive. This shows the
range of questions and requests that the SFFP Education Assistants As attend to
on a regular basis. Several innovative activities implemented by the EAs deserve
special mention here. With active involvement of LUCE's (Lincoln University
Cooperative Extension) State Marketing Specialist, the Program assisted
African-American small farmers to launch a "pastured poultry"
operation in the Southeast area of the state. One collaborator raised and sold
more than 400 birds last year generating income for his family. In the Southwest
part of the state, another EA helped a small farm family start a small fruit
operation. This operator now has an established orchard where the buyers come
and "pick their own," or walk into the family store and purchase
freshly picked berries and/or fruit preserves. The centerpiece of the Program's
many success stories last year must have been the "Green Kids Gardening
Project." One SFFP-EA and a UO/E 4-H Regional Specialist conceived the
idea, and with financial support from LUCE and volunteer support from the
participating communities, the Project was implemented in Butler County. Four
community gardens were established, one in each collaborating community, in
which more than 150 individuals participated (90 percent belonging to age group
5-14). The communities were predominantly African American, and overwhelming
majority of the participants came from the low-income minority families. In
addition to gardening activities, the participating kids were able to attend a
summer camp, which was probably a first and only opportunity for many of them.
This Project will continue in 2001 with increased community participation.
Sheep cheese production as a value added product was introduced
to several Missouri Sheep producers. One such farmer is now a certified sheep
cheese producer. Work is being done to expand this needed niche market so that
they will be able to sustain themselves economically. Contracts were made with
15 possible market outlets in northwest Missouri for expanding the market.
A third annual Missouri Goat Marketing Conference was held to
provide educational materials to small-scale producers. Information at the 2000
Conference concentrated on production practices without the use of hazardous
chemicals. In the course of presentations at conferences for vegetable, goat,
and sheep producers, attendees were presented with the implications of the
present global economy and market to what they are producing in agriculture.
Efforts to explore the opportunity of growing and packaging vegetables for
external markets are continuing with a group of 25 farmers being helped to
develop expanded vegetable and pastured poultry operations.
During FY2000, the horticulture programs at Lincoln University
distributed the Vegetable Production Newsletter to more than 2300 growers and
stakeholders in the vegetable industry in Missouri. The fourth annual Great
Plains Vegetable Conference was held in St. Joseph, Missouri with more than 450
growers, marketers and others related to the vegetable industry in attendance.
This conference is the joint effort of vegetable specialists at Lincoln
University and within Missouri and others within the states of Kansas, Nebraska
and Iowa. The crop nutrition program has worked with more than 200 fruit and
vegetable growers within the state. Growers on this program report increased
yield, quality and consistency of produce, and increased profits. For example,
one peach grower in southeast Missouri reported that his return per acre was
$500 greater than at any time in the history of the orchard, and the orchard has
been in business for more than 30 years. Lincoln University has contributed
significantly to the Master Gardener Program in Missouri by offering training
and assisting with the coordination of the programs statewide and especially in
collaboration with the Missouri Botanical Garden in St. Louis. This Master
Gardener program alone has more than 200 volunteers that assist with
horticulture needs in the greater St. Louis area.
Six new video titles were developed and used as a training tool
for small limited resource farmers in the areas of sheep husbandry and value
added wool production.
An additional 5 guide sheets were produced on subjects relating
to sheep and goat production.
Outreach Technical Assistance Program (2501)
Through our project's endeavors, one of Missouri's minority
owned banks extended its interest in lending through the FSA Guaranteed Program,
to more African-American farmers in the Bootheel. Our farmers were made aware of
this lending opportunity and consider this source crucial for options in
agricultural lending. Through a meeting with Lincoln University, Gateway
National Bank, and the USDA Office of Outreach Review Team, new ideas and
opportunities were spawned with Rural Development programs and Lincoln
University Cooperative Extension that will bring new rural housing and value
added agriculture opportunities to Southeast Missouri.
Project staff serves as advisory committee members to a
collaborative-formed woman’s agricultural group. Outreach to this 60-member
organization is targeted in the form of Lincoln University's position on the
committee. Three presentations on LU Extension programs and outreach effort were
given, as well as updates in the monthly meetings. The group now looks to LU for
University Extension support and is made aware of the many programs available.
Many women who participate are actually "operating" their farm
including one member who became New Madrid County, "Farmer of the
Year." Groundwork for a second Agri-women project is being developed by the
Dunklin County NRCS staff.
Meetings with the faith-based "Adopt-A-Farm-Family"
Coalition have opened opportunities to assist farm families with financial
assistance and advise in areas of farming such as lending, tax information,
legal matters and such. Project staff has a position on the board of the
organization. In addition, farm families in need of "living monies ' are
referred to this group.
Our staff has successfully enriched our clients by bringing them
together to attend conferences and workshops. Participation in the USDA
Agricultural Marketing and Outreach Conference proved successful as a networking
opportunity, as well as providing new information and programs for our farmers
to bring home. USDA staff at the Memphis, Tennessee Farmers Conference commented
on our farmer's awareness and education on agricultural issues, which lead to a
local paper printing an article on LU's farmers.
Office of Outreach conducted a review of our project that proved
successful and merited praise from the agency. The review team consisted of
representatives from RD, FSA and NRCS. Through the review, successes were
outlined that reflected on the project's initiation of the NRCS Vegetable
Cooperative as well as our unique collaboration with Gateway National Bank.
GOAL 2: To provide a safe and secure food and
fiber system
Programs conducted under this goal addressed: Food Quality, Food
Handling, Food Accessibility and Affordability.
Nine hundred thirty-two (932) home visits were conducted by
nutrition paraprofessionals working in the six counties of southeastern Missouri
(Bootheel). As a result of these home visits: 131 participants indicated that
they practiced safe food handling practices more often than before the visits,
including hand washing before handling food, refrigerating food promptly after
meals, and keeping raw meats separate from other foods.
Ten (10) cooking classes were conducted in Jefferson City,
Missouri, emphasizing the importance of eating fruits and vegetables and
preparation of delicious low-fat, low-sugar meals and snacks. A sample of those
attending volunteered that they would try these recipes at home and asked for
copies to give to family members.
Twenty five (25) workshops and conferences were held on methods
to develop a secure food and fiber system.
GOAL 3: To achieve a healthier, more
well-nourished population
Programs conducted under this goal addressed the following
themes: Human Health and Human Nutrition.
Workshops on after school nutritious snacks and activities
related to the Food Guide Pyramid were conducted with 88 youth in after school
programs. In one program, the youth drew pictures depicting what they had
learned. Most of the pictures indicated that they understood the importance of
eating more fresh fruits and vegetables. When they have a chance to make these
kinds of snacks themselves, youth are more likely to eat them.
The Food Stamp Nutrition Education Program (FSNEP) staff reached
124 adults through-group presentations. The groups consisted primarily of
African Americans, ranging in age from 19 to 88, with an emphasis on
grandparents raising or providing day care for grandchildren. It is important to
work with this group because of their influence on the diets of the young
children in their care. In addition to home visits and the group presentations
mentioned above, the FSNEP paraprofessionals staffed a booth at several health
fairs and handed out relevant nutrition information as well as literature about
the FF-NEWS program.
In FY 2000, the Specialist presented talks on "Trends in
the American Diet," "Health at Every Size," and "Cooking for
Health." Participants volunteered that they found the information presented
useful in helping them to make dietary changes.
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There were 25 workshops given in the area of food and
nutrition to 300 participants.
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There were 300 fact sheets distributed.
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One hundred fifty program participants demonstrate having
the knowledge, skills and ability to be well nourished.
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One hundred twenty-one program participants showed much
progress with food preparation skills.
GOAL 4: To achieve greater harmony between
agriculture and the environment
Programs conducted under this goal addressed the following key
theme: Sustainable Agriculture.
Pastured poultry operation began in Neelyville, MO in June 1999.
The Lincoln University Cooperative Extension marketing program, the small farm
program, and the extension unit as a whole undertook this effort. The practice
of pastured poultry, apart from its enhancement of the economic well being of
the small farmer, prevents any adverse impacts that ongoing indoor mass
production of chicken may have on the environment.
Within this fiscal year, the farmers have produced five batches
of chickens totaling, approximately, 1,000 birds. A profit of $4.00 is made per
chicken weighing an average of 6 lbs.
GOAL 5: To enhance economic opportunities and the
quality of life between families and communities
Programs conducted under this goal addressed the following key
themes: Agricultural Financial Management, Impact Change on Rural Communities,
and Supplemental Income Strategies, Children, Youth and Families at Risk,
Communications Skills, Jobs/Employment, Leadership Training and Development,
Promoting Business Programs, Workforce Preparation for Youth and Adults, and
Youth Development/4-H.
The marketing programs were designed to improve the prices
farmers were receiving for their farm products. These have proved successful and
farmers are very happy to increase their share of the dollar that a consumer
pays at the marketplace. With the prospect of increasing their income, producers
are eager to now expand production for economic sustainability on the farm. The
areas of marketing include Internet auction sales of Missouri goats and
community-assisted pastured poultry marketing.
Eight workshops were designed to provide development of careers
and/or life skills. Participants gained knowledge and skills need to promote
economic opportunity for the disadvantaged in Missouri. Output indicators
indicated more than 300 youth and adults completing programs and providing
feedback on the programs.
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Seventy learners participated in Lifeskills workshops
offered to FUTURE clientele.
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Eighty clients participated with Weed and Seed's "Never
Too Late" workshop designed to increase self-esteem and encourage
effective parenting.
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Bootheel youth participated in 121 workshops in the area,
and across the state designed to provide career development and Lifeskills
including self-esteem, alcohol and drug prevention, sexual abstinence,
conflict resolution, decision making, etc.
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More than 6,955 program contacts were made to l500 youth and
families.
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Forty youth participants learned that jail is not a place
that they would want to ever go.
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One thousand five hundred youth learned that abstinence and
safe sex are ways to prevent unwanted pregnancy and STDs.
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One thousand five hundred youth and adults acquired
knowledge about the negative impact of alcohol and drug usage.
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Fifty eight youth and adult participants acquired knowledge
relating to business development and entrepreneurship.
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One thousand youth were exposed to college and career
exploration workshops.
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Eleven youth acquired hands on experience in gardening.
Accomplishments for the Community Development
Program (CDC)
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Selection of an Advisory Board.
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Obtained 501 C (3) Status.
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Conducted Needs Assessment and Development of a 5-year
strategy plan.
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Identified potential jobs and organize training to match
jobs.
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Eight (8) residents received their "Lead
Abatement" license from the State of Missouri; several loan
applications; have been made by qualified homeowners in Hayti Heights
through the Partnership of the CDC, the Home Loan Mortgage Corporation
(HLMC) and the City of Hayti Heights.
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Project pending implementation included the establishment of
a local office of HLMC, thereby creating jobs in Hayti Heights.
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Worked with North Lilbourn community to provide residents
with affordable housing.
Missouri Institute on Minority Aging
The Annual Missouri Institute on Minority Aging is a cooperative
agreement between the Missouri Department of Social Services, Division of Aging
(DA); Department of Health, Office of Minority Health (DOH); Department of
Mental Health (DMH); Office of Administration, Office of Equal Employment
Opportunity (OA); and Lincoln University of Missouri (LU). The Minority
Institute is in the form of a conference consisting of educational workshops,
speakers, and programs focused on minority aging and health issues. In 2000,
more than 200 state and local agency professionals in the fields of aging and
health; academic professionals with a focus on aging and health-related topics;
and aging consumers received training by minority health professionals and
Lincoln University Cooperative Extension specialists. The majority (63)% of
participants were social workers from the Division of Aging and represented 35
counties in the state. Workshop topics included diversity training; improving
diabetes care for seniors and high-risk populations; race, age, and the 2000
census; chronic disease issues and challenges for minority elderly populations
in Missouri; outreach with diverse populations and groups; dental care for
minority aging populations; and, how senior centers can help minority aging
populations.
As a result of attending the Lincoln University Cooperative
Extension sponsored training, 90% of participants reported feeling that the
Minority Institute extended their knowledge of the social, economic and health
issues impacting older minority individuals, as well as resources available in
the state for older minorities. The knowledge gained by participants has the
potential to improve the delivery of health care services and/or improve the
quality of life for minority older individuals. In every workshop, more than 75%
of participants reported that the content of the workshop provided them with
information helpful to their work. Comments such as "I will go back home
with more good news to share" and "I learned something that I can take
back to my city" were common in evaluations.
In addition, the Minority Institute impacted the racial
attitudes of the participants. The great majority of participants (85%) reported
that the diversity training was helpful to their current work and report feeling
more knowledgeable of ways to communicate with different cultures. The potential
to change racial attitudes is reflected in the following comment made by a
participant: "I'm on the diversity training committee at work and also
supervise a team of African American staff. It has been a confrontation between
what I believe and what I do. The (diversity training) really helped me to
understand where my /our difficulties in communicating arise. I will share the
information with a lot of people."
Computer Literacy and Entrepreneurial Skills
Training Project
A cooperative agreement between Lincoln University Cooperative
Extension's Missouri Center on Minority Health and Aging and the Missouri
Department of Social Services, Division of Family Services makes it possible to
build human capital resources by providing computer literacy and entrepreneurial
training to TANF recipients, underemployed and unemployed persons in Cole
County. Low-income, single mothers, and African American males are key audiences
targeted by this program.
In 2000, more than 120 individuals from Cole County received
computer literacy training by Lincoln University Cooperative Extension staff.
Approximately half the individuals served are unemployed while an additional 40%
are under employed. African-Americans represent 40% of those who are considered
under employed.
The computer literacy training is a ten-week course and covers
introductory lessons on Microsoft Office and its applications. An additional 33
individuals received entrepreneurial training. Of those served, the majority is
female and one third are African American.
Over (100) individuals thus far have completed the ten-week
computer literacy training and earned a certificate of completion. As a result
of attending the Lincoln University Cooperative Extension sponsored training,
limited resource individuals have gained relevant job skills and vocational
training in computers. Specific skills have been gained in Microsoft Office
word-processing, databases, and navigating the worldwide web. Participants
report feeling more confident using computers, as well as interest in attending
more training. The course also has the potential of increasing self-confidence
of welfare-to-work participants, as expressed in the following statement
submitted with evaluations: "You all have helped me so much and I'm proud
of myself thanks to you." Three (3) individuals who completed the
entrepreneurial training wrote a business plan and were successful in starting
their own business.
Youth at Risk
Over 350 youth-at-risk and underserved adults participated in
the National Youth Entrepreneur Symposium hosted by Lincoln University and held
in St. Louis this program year. Youth learned the knowledge and skills needed to
be a success in business. They participated in over 18 workshops, skill
development seminars, model programs, business exhibits, panel presentations,
mentoring programs, and hands-on practical experience.
During the 2000-2001 program year, the focus of the parenting
education efforts was to support a Father's Rites of Passage program in St.
Louis, Missouri. Approximately 45 fathers participated in parenting programs
provided by Lincoln University through a partnership with the Urban Male
Resource Center.
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Two hundred (200) youth ages 5-16 participated in 46
sustained educational non-school hour programs in public housing and
Juvenile Detention.
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Thirty-two (32) youth gained knowledge in food safety and
the prevention of food borne illnesses
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Four (4) youth gained leadership skills, serving in youth
leadership roles.
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Twelve (12) youth gained skills and knowledge in
understanding the differences between love and infatuation. This is a
critical step in the reduction of teen pregnancy.
Lincoln University Region, School-to-Careers
Lincoln University Region School-to-Careers Coordinator
continues to work with the 36 school districts within the designated eight
county region. Much of the work for the 2000 fiscal year has been orienting many
of the schools to the benefits of the School-to-Careers program.
Programs continue to be offered to schools within the Lincoln
University Region. The most popular program in 2000 was the collaboration
between Lincoln University and Linn State Technical College who provided
educators the opportunity to participate in teacher internships. Approximately
59 educators participated in this program. Educators are offered a $1,000.00
stipend and college credit for this internship. Educators are then required to
submit lesson plans detailing how they will implement what they learned while
completing the internship. This lesson plan is utilized in the classroom to
allow students the opportunity to learn from actual work experience.
Job Shadowing has been another popular activity for students to
gain hands on information as to how their schoolwork applies in the field of
work. On February 2, 2000 there were 706 students who were hosted by 328
businesses within the Lincoln University Region School-to-Careers program.
The Lincoln University Region has also had the opportunity to
support professional development for teachers within the region. Most recently
the Lincoln University Region sponsored a speaker to attend the Jefferson City
Public Schools Staff Development Day. In an effort to provide teachers with
strategies to support students at risk of failure, programs were conducted that
address youth at risk issues.
END - Lincoln University Cooperative Extension
Programs AREERA Accomplishment Report FY 2000
Cooperative Research Programs
The 1999-2000 research program at Lincoln University focused on
four main areas: animal science, crop science, human nutrition, natural
resources and agricultural economics. The 1999 Plan of Work approved projects
addressed the following broad national goals adopted by the National
Agricultural Research, Extension, Education, and Economics Advisory Board:
Goal 1: An agricultural system that is highly competitive in the
global economy.
Goal 3: A healthy, well-nourished population
Goal 4: An agricultural system which protects natural resources
and the environment.
All but two of the projects reported here reached their five
year completion dates in 1999 and 2000. The remaining two will end September 30,
2001. A revised five year plan of work follows this report.
Research conducted under this goal addressed the following key
themes: Agricultural Profitability; Animal Health; Animal Production Efficiency
Plant Production Efficiency, Aquaculture and Small Farm Viability.
At least five manuscripts have been published in professional
journals related to this goal area.
MOX-OC94-610
Fungicide and Soil Interaction Effects on Plant Growth and
Development
Accomplishments and Results
The influence of fungicides on soil microbes and plant growth
was evaluated in experiments conducted from 1994 to 1999. It was found that
certain fungicides reduce the Biodiversity (i.e.. reduce the number of different
types) of microbial populations in the rhizosphere (i.e. the soil close to the
roots of plants). In addition to reducing the diversity of soil microbes, the
percentage and number of phytotoxic microbes, primarily bacteria, increase with
the use of certain fungicides. Phytotoxic microbes may have the potential to
inhibit normal growth and development. These non-target effects of these
fungicides strongly suggest that there may be major concerns with the repeated
and continuous use of pesticides that can alter soil microbial populations,
especially if these prove to be long-term effects. This work also gives possible
explanations for reduced productivity reported in tropical and subtropical crops
where pesticides are applied year round. While fungicides are designed to target
specific pathogens, their impact on microbial communities may have unexpected
consequences. This work indicates the need for not only evaluating pesticides
for the efficacy to control target pests, but also their other effects on the
environment, especially soil microbial communities.
MOX-OC94-611
Sweet Potato Production in Central and Southeast Missouri
Accomplishments and Results
Field plots were established in Southeast Missouri to
investigate the effects of soil applied nitrogen fertilizer, irrigation and time
of harvest on sweet potato (Ipomea batatas) growth and development. Four
rates of urea fertilizer, five irrigation periods and five sweet potato
varieties were arranged in a completely randomized split-split-split plot design
at Lilbourn, Missouri. Sweet potatoes were transplanted on or about May 15 of
each year of the study. Plant samples were taken at 90 and 120 days of growth.
Field data collected included rainfall air temperature, soil temperature, soil
moisture, leaf area, and fresh weight of vines and roots. Root and vine dry
matter, percent leaf nitrogen, marketable root yield and number of roots were
determined in the laboratory. Total amount of water supplied during each period
of irrigation was measured. Data preparation and statistical analyses are
underway at this time.
Overall Program Impact
This research investigation was conducted on a farm site owned
by the Delmo Cooperation in Southeast Missouri. It not only provided an
opportunity to collect information of value to producers and consumers, it also
served as an education resource and demonstration for people in the surrounding
communities. We are aware that at least one farmer decided to grow sweet potato
crops as a result of his exposure to this research. The Cooperative Extension
Service often used the site for demonstration. The Principal Investigator
conducted numerous tours of the site for different groups during the course of
the study. Upon completion of the data analyses, we will be able to provide
farmers and extension personnel with better information on management practices,
yield expectations, water use efficiency and product quality. The sweet potato
is more attractive as a crop to the small-scale farmer than the large-scale
farmer.
MOX-OC95-612
Influence of Farming Systems on Selected Soil Quality
Parameters
Accomplishments and Results
Experiments were repeated for 3 years (1996-1999) at Lincoln
University Carver Memorial Farm using silty clay loam soil to study the
influence of different herbicides, in combination with tillage systems, on soil
quality parameters. Soybean and corn cultivars commonly used in central Missouri
were planted and were treated with pre- and post –emergence herbicides during
the growing season. Selected chemical and microbiological characteristics of
soil samples taken from experimental plots were measured. There was consistent
decrease in soil pH with depth but no significant change due to tillage or
herbicide applications. However, results indicated significant reduced activity
for several important soil enzymes (glucosidases, phosphatases, urease, and
sulfatase). Our findings complemented studies by other researchers in using soil
enzyme activities as an index to monitor soil quality in relation to pesticide
pollution of soils.
MOX-OC97-613
Improving Seedling Performance of Warm Season Vegetables
at Low Temperatures
Project MOX-OC97-613, Improving Seeding Performance of Warm
Season Vegetables at Low Temperatures was completed on December 31, 1999 and the
accomplishments as outlined in the AD-421 termination report were as follows.
Accomplishments and Results
Tissue culture studies were conducted with two warm season
vegetables to facilitate their improvement. Cotyledons of young cowpea (Vigna
unguiculata) embryos from four genotypes were tested for their ability to
produce somatic embryos. Several media combinations using 2,
4-dichlorophenoxyacetic acid (2,4-D) and Murashige and Skoog basal salt mixture
(MS) with B5 vitamins were tested. The cotyledons produced shoots and several
somatic embryos following subculture. Most of the embryos were at the globular
stage and did not mature. Other cultures of cotyledons of mature seeds grown on
Gamborgs medium with benzylaminopurine produced shoots. Globular somatic embryos
were produced from okra (Abelmoschus esculentus) cotyledons and
hypocotyls cultured on MS with 2,4-D. Cowpea genotypes evaluated at 14/100C,
15/100C and 16/100C, day/night temperatures varied in seed
germination and seedling characteristics.
Overall Program Impact
This project provided information to facilitate the
improvement of seedling performance at unfavorable chilling temperatures.
MOX-OP96-416
Effect of Chlortetracycline on Postpartum Fertility &
Pregnancy Rate in Early Postpartum Ewe
Accomplishments and Results
Reproductive efficiency is a problem in small ruminants. To
achieve two lamb crops per year the ewe must be bred within thirty-five days
after lambing. When ewes are bred naturally before thirty-five days postpartum
fertility and lambing rates are very low. However, when day 32 postpartum ewes
are intrauterine inseminated, fertility rate increased to 60% thus demonstrating
other factors adversely affect sperm transport when the day 32 postpartum ewe is
bred naturally. Research at Lincoln University has shown that fertility
increases as the length of the postpartum interval increases. Decreasing the
postpartum interval will provide the small ruminant producer an opportunity to
increase production in a shorter period of time.
Recently, it has been demonstrated that feeding
chlortetracycline (CTC) to beef heifers with reproductive-tract infections (1.1
mg/kg/day) for 30 days prior to the breeding season or to beef cows
(.5g/head/day) starting at 42 days postpartum for 14 days resulted in an
increased pregnancy rate. Sows that were fed low levels (.4 g/day) of CTC from 1
week before the initiation of the breeding season to 15 days after the breeding
season and high levels (1.2 g/day) of CTC from 110 days of gestation through
lactation improved overall reproductive performance. Feeding CTC seems to
improve reproductive performance in other species, but it is not known what
effect CTC has on fertility and pregnancy when fed to small ruminants during the
first twenty days postpartum. The objectives of the following experiments were
to determine the effect of chlortetracycline (CTC) on fertilization rate
(Experiment 1) and embryo survival in the early postpartum uterus of ewes
(Experiment 2). Postpartum ewes were divided into the following treatments: 1)
fed a grain ration without CTC (controls); and 2) fed CTC (500 mg/animal/day)
six weeks prior to lambing until day 22 postpartum. Ewes received a vaginal
sponge (40 mg of progesterone) on day 9 postpartum . Nine days after receiving
vaginal sponges, they were removed and ewes were given an injection of 750 IU
PMSG to induce estrus. Ewes were checked for estrus twice daily beginning 36
hours after PMSG injection. At estrus or 48 hrs post PMSG injection, ewes were
inseminated with .2 ml of mixed semen, collected from two rams, into the tip of
the uterine horns. Two days post estrus or 4 days post PMSG injection, ewes were
laparotomized and oviduct(s) on the side of ovulation were flushed for ova
recovery. Ova were recovered from 84% of CTC ewes (n=56) and 75% from the
controls (n=60). Of the ewes from which ova were recovered (CTC= 47; controls=
45), 72% of CTC ewes had fertilized ova (P<.05) compared to 31% of control
ewes. In Experiment 2, thirty-two ewes were divided equally into the same
treatments as in Experiment 1. Twenty-five days post insemination, ewes were
checked for pregnancy with an ultrasonic scanning monitor. Ewes were allowed to
go to term. The percentage of CTC ewes (50%) that were pregnant at day 25 post
insemination was not different (P>.05) from controls (33%). By day 30 post
insemination none of the ewes maintained their pregnancy. These data suggest
that fertilization rate is improved in ewes on postpartum day 20 by feeding CTC
during late gestation and early lactation and CTC had little effect on embryonic
survival in the early postpartum uterus.
Overall Program Impact
Fertilization rate of the early postpartum ewe can be
increased by feeding chlortetracycline, but the number of pregnant early
postpartum ewes still remains low. Determining different mechanisms or factors
involved with fertility and pregnancy in the early postpartum small ruminant
will provide ways to increase reproduction. This will allow the producer to
increase their production by having
MOX-OP97-417
Activation and Atresia of Primordial Follicles
Accomplishments and Results
Improved understanding of the early stages of follicular
growth would allow more effective utilization of the approximately 100,000
oocytes found in a single ovary of mature cows. Current technology allows use of
only a fraction of these oocytes which impedes rapid genetic improvement by
exceptional females. Development of in vitro techniques to manipulate
growth of these follicles has been disappointing, with the majority of
primordial follicles disappearing and few follicles progressing beyond the
primary stage of development. The hypothesis tested this year was that ovaries
could be removed from heifers and transplanted into SCID mice, for continued
development. Successful transplantation of ovaries into mice would also allow
examination of bromodeoxyuridine (BrdU) uptake into granulosa cells of
follicles. BrdU could then be used to determine how long follicles remain in
early stages prior to growth.
Ovaries were successfully transplanted and were healthy for
the 28 days that they remained in the SCID mice. However, results were
disappointing because essentially all primordial follicles disappeared by the
end of the transplantation period. The majority of follicles remaining in the
ovarian sections were early primary (a mixture of squamous and cuboidal
granulosa cells in a single layer) and primary (only cuboidal granulosa cells in
a single layer). It was promising that the remaining follicles continued to grow
following transplantation and some advanced as far as the secondary follicle
stage. The suriviving small follicles (early primary and primary) were healthy
and showed growth and division of granulosa cells as measured by incorporation
of BrdU into the DNA. In conclusion, these results demonstrate that ovarian
sections can be transplanted successfully under the kidney capsule and follicles
will continue to grow. However, due to loss of primordial follicles, and the
expense of purchasing and maintaining SCID mice, alternative methods for study
of small follicles in bovines should be pursued.
Overall Program Impact
It was demonstrated that there continues to be significant
technical barriers that must be eliminated before it is possible to successfully
manipulate the 100,000+ small follicles contained in ovaries of cattle. Once a
procedure is successfully developed, oocytes can be matured in vitro, fertilized
with the appropriate semen, then stored frozen until used. This provides a
method that limited resource individuals can upgrade the genetics of their herd.
MOX-OP92-803
Establishing a Research Support Capacity at Lincoln
University for Missouri Aquaculture Producers
Accomplishments and Results
Current Situation: Missouri leads the north central
region of the United States in sales of fish. Currently, however, there is no
formalized aquacultural research program within the state. There is tremendous
potential for expansion of this industry in Missouri. The reasons include the
central location of Missouri within the United States and the fact that the
state is a major site for aquacultural production. There are also fewer
legislative restrictions in Missouri than in other states.
The objective of this project is to build capacity to conduct
aquaculture research at Lincoln University. The project involves surveying
production problems; conducting on-farm evaluation of bird and mammal predation
problems and determining effects of federal and state policy issues related to
aquaculture.
A survey instrument was developed and sent to eighty-four
aquaculture producers in the state. At this point forty-six have been returned
and entered into a database. A statistical analysis has been completed. The
survey results indicated that 79% of aquaculture producers had problems with
bird depredation and 66% had problems with mammal depredation. State fish
hatcheries were also surveyed and showed that 100% of the hatcheries had
problems with bird or mammal depredation. At this time eleven private producers
have gone out of business in the past three years.
All state and federal regulations effecting aquaculture
producers have been reviewed. The number of agencies and sub-agencies alone give
reason for confusion. There are also county and local regulations that effect
aquaculture farmers. All of these areas need to be addressed and controlled,
however, compliance with all the regulations can be costly, time consuming, and
distracting to the typical aquaculture farmer.
A document of the policies, regulations, and permits
pertaining to aquaculture farmers in Missouri has been developed along with a
flowchart to assist farmers in understanding their obligations concerning
federal and state regulations. The documents and flowchart allows for assistance
on any individual aquaculture farm in Missouri. Publication of this information
will be very helpful to our clientele.
Research conducted under this goal addressed the key themes
of Human Health and Nutrition.
Two manuscripts have been published in professional journals
and seven professional presentations related to this goal area have been made.
One presentation will be made on April 2, 2001 at the annual Experimental
Biology Conference in Orlando Florida.
MOX-OH98-528
Dietary Fat, Antioxidants and Lipid Peroxidation
Accomplishments and Results
The effects of dietary fats and antioxidants on blood
pressure and plasma lipids were investigated in spontaneously hypertensive rat
model. Animals were fed diets containing 10% fish oil or 10% beef tallow with
three different antioxidant levels. The control diets (both fish oil and beef
tallow diets) contained 50 mg vitamin E and 2 mg beta carotene per kg. The
medium antioxidant diets contained 400 mg vitamin E, 200 mg vitamin C and 200 mg
beta-carotene per kg. The high antioxidant diets contained 800 mg vitamin E, 400
mg vitamin C and 400 mg beta-carotene per kg. Diets and water were provided ad
libitum for 12 weeks. Increases in systolic blood pressure significantly
attenuated in rats fed the fish oil diets as compared with rats fed beef tallow
diets. Dietary antioxidant supplementation reduced blood pressure only in rats
fed the fish oil diets. Plasma triglycerides, total cholesterol and HDL-cholesterol
were significantly reduced in rats fed the fish oil diets as compared with rats
fed the beef tallow diets. Dietary antioxidant supplementation did not affect
plasma lipid levels. It is concluded that dietary fish oil is effective in
lowering blood pressure and plasma lipids. Dietary antioxidants appears to
enhance the hypotensive effects of fish oil.
Overall Program Impact
Essential hypertension is a major health problem in the
United States, affecting more than 23 million adult Americans. It is of
particular concern to certain minority population such as African-American. It
is estimated that one- third of the African-American adults have high blood
pressure.
The information generated from the hypertension project can
be used for diet modification to prevent development of hypertension in people
at high risk and can be applicable as an adjunctive therapy for hypertension
because of their safety and considerable economic savings.
MOX-OH97-525
Dietary Protein Restriction, Running Activity, and the
Development of Obesity
Accomplishments and Results
The primary focus of this project was to examine how diet
composition influences voluntary running activity in obese and non-obese animal
models. The study confirmed our previous work demonstrating that reduced protein
intake blocked development of obesity in an obese animal model, the hypothalamic
obese rat. The results suggested that this was not due to increased physical
activity in this obese animal model. In contrast, dietary protein restriction
caused increased running activity in non-obese rats and the results indicated
that this response was gender specific, occurring in males but not in females.
These results suggested that energy balance responses to different dietary
protein levels is gender specific, and that different dietary recommendations
for weight reduction and prevention of obesity and other eating disorders may be
necessary for males and females.
Key Program Components
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The influence of diet on energy metabolism, obesity and
eating disorders.
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The influence of diet-induced obesity on hypertension.
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The influence of antioxidants on lipid peroxidation.
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The influence of vitamin A and carotenoids on health of
minority populations.
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The influence of fat intake on health of elderly.
Overall Program Impact
Results of this study have identified dietary factors
involved in development of obesity and will contribute to dietary
recommendations for prevention and treatment of obesity. Reduced incidence of
obesity in the United States will reduce total economic costs and direct medical
costs associated with obesity-related chronic health problems such as
hypertension, type II diabetes, and cardiovascular diseases. Currently, these
annual costs are $99.2 billion and $51.6 billion, respectively.
MOX-OH97-527
Development of Culturally-Sensitive Carotenoid Database
for African Americans
Statement of Issue(s):
The higher incidence of mortality rates for cancer in African
Americans than in white Americans or members of other minority groups have been
well documented. Despite the higher risks of dying from cancer, few data on diet
and cancer in African Americans are available. Carotenoids in dark green and
yellow fruits and vegetables have shown health benefits to humans by inhibiting
cancer development. Using the datbase developed by this project should provide a
better understanding of the role of carotenoids in cancer development in African
Americans.
Objectives:
1. To explore traditional African American diet and food
practices through focus group interview.
2. To analyze carotenoids in foods which are unique to
African Americans ans lacking reliable data in the USDA carotenoid database.
Accomplishments and Results
The purpose of this project was to develop a
culturally-sensitive carotenoid database containing specific entries for
traditional foods consumed by African Americans. The USDA-NCI Carotenoid
Database was used as a blueprint. Data of the focus group in Southeast Missouri
showed that greens, corn, tomatoes, cabbage, and green bean were the most
frequently mentioned vegetables. A survey of the fruit and vegetable consumption
pattern of 58 African Americans living in the Bootheel and St. Louise areas of
Missouri was conducted. Preliminary analysis of the survey data indicated that
mustard greens, turnip greens, collards, green beans, baked beans, corn,
tomatoes, fried and baked potatoes, mixed vegetables, and green salad were the
frequently consumed vegetables. Bananas, apples, oranges, and watermelon (in
season) were the frequently consumed fruits. Berries (except strawberries) such
as blueberries, blackberries, raspberries were seldom consumed. Spinach, alfalfa
sprouts, cauliflower, and brussels sprouts were those vegetable seldom consumed.
These preliminary findings shed some lights about the fruit and vegetable
consumption patterns of African American in Missouri.
Key Program Components:
The USDA-NCI Carotenoid Database developed by the USDA Human
Nutrition Research Center at Beltsville; minority groups, the elderly, low
income populations, and other under-represented populations.
Overall Program Impact
Results from this study provided valuable information for
developing more effective intervention programs that promote healthy dietary
habits and reduce risks of cancer development.
Research conducted under this goal addressed the key theme of
nutrient management. Three professional presentations related to this goal have
been made.
MOX-OP98-418
Nitrogen & Phosphorus Excretion in Growing Pigs:
Effect of Phase Feeding Fiber & Phytase
Accomplishments and Results
A study involving three week-old pigs (n= 84) was conducted
to evaluate the efficacy of intrinsic phytase (IP) enzyme from wheat middlings,
and the combined effects of phytase, dietary fiber, and phase-feeding of dietary
proteins on fecal ammonia (NH3), and volatile fatty acids (VFA)
emission, and weight gain of growing pigs. The pigs were housed in groups of
7/pen, with dietary treatments randomly assigned to pens (3 pens/diet) with pens
serving as experimental units. The diets used were: corn soybean meal-based
control diet (D1), D1 plus microbial phytase (MP; Natuphos, 1,200 PU/g; D2), D1
plus intrinsic phytase (IP; D3), and IP and MP combination (D-4). The diets were
also designated as low (1 and 2), or high (3 and 4) fiber diets. Feed was
offered once/d on a regular (D1, D3) or, phase-fed (D2, D4) basis with free
access to water. Individual pig weight, and pen feed consumption were measured
weekly. Fecal samples collected from each pen were used for VFA, and NH3
analysis. Results from the study indicated that pigs that were fed diets
containing phytase enzyme consumed more feed (P< .05), gained faster
(P<.05), and were more efficient (14.5%) than the pigs that were fed control
diet. Total, and individual VFA in feces from pigs fed low fiber diets were
higher (P<.05) than with high fiber diet fed pigs. Concentration of NH3
in feces from pigs fed low fiber diets was higher (47.6%; P<.05) than in
feces from pigs fed high fiber diets. However, the level of NH3
within each fiber category was not affected by the treatment. Microbial phytase
supplement of the corn soybean meal-based diets (2.5 kg/ton), or substituting
25% wheat middlings for an equal part corn in the corn soybean meal-based diet
resulted in improved feed intake, feed efficiency, and growth performance of
pigs, and significantly reduced the concentrations of ammonia, and VFA in feces.
Overall Program Impact
Knowledge concerning hind-gut fermentation in pigs is
relatively scarce and most of what is know today is extrapolated from ruminant
animals. Also, in the face of the ever growing public concern regarding swine
odors, the fermentation processes in the hindgut especially when
non-conventional feedstuffs are fed, or when feedstuffs are manipulated to
improve efficiency, need to be thoroughly investigated.
Substituting 25% of the corn in the corn soybean meal-based
diet of growing pigs with wheat middlings did not adversely affected feed
intake, or feed efficiency of pigs but average daily weight gain was
significantly reduced. The mechanism for the improved feed intake and feed
efficiency is not clear. However, the increased availability of phytate bound
calcium and phosphorus from the diet, and the potential for the development and
maintenance of a healthier gut tissue that may have resulted from the inclusion
of fiber in the diet, and the consequential improvement of nutrient absorption
by the wheat middling fed pigs may have contributed to the improvement. Results
from the study suggest that the comparable animal performance observed between
phytase supplemented low-fiber, and high-fiber diet fed pigs may have been the
result of the additional intrinsic phytase from wheat middlings, which may have
contributed to the improved fermentation capacity of the microorganisms in the
hindgut and the overall growth performance of the pigs. The results of our trial
has also clearly indicated that ammonia (NH3), and volatile fatty
acids (VFA) emission in feces from pigs fed 25% wheat middling was significantly
reduced. This may not only indicate a degree of improved nutrient utilization by
pigs but a significant reduction of the potential odor producing organic
compounds in feces. Therefore, partial substitution of the diet of growing pigs
with wheat middling can reduce production of organic compounds generally
associated with swine odor but weight gain of pigs will be reduced if the energy
value of the diet is not increased. Wheat middlings is a relatively cheap feed
ingredient, and the improvement in N and P utilization, as reported in earlier
studies, and the superior growth performance of pigs fed diets supplemented with
25% wheat middlings with/without microbial phytase in the current study may
serve as an economic incentive to include it in the diet of growing pigs.
However, more research is needed to define the optimum levels of wheat middlings
that can be included in the diet of growing pigs.
END - Lincoln University Cooperative
Research Programs AREERA Accomplishment Report FY 2000
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